Journal: Cell Stress & Chaperones

Article Title: Effects of cobalt chloride on the stem cell marker expression and osteogenic differentiation of stem cells from human exfoliated deciduous teeth

doi: 10.1007/s12192-019-00981-5

Figure Lengend Snippet: The cytotoxicity of CoCl2 was measured. a Cell viability of SHEDs was determined using a CCK-8 assay after incubation with or without 50 or 100 μM CoCl2 for 1, 3, 5, and 7 days. The absorbance was measured at 450 nm. b The percentage of STRO-1+ cells in SHEDs after incubation with or without 50 or 100 μM CoCl2 for 3 days. The data are shown as relative fold changes compared with the control. c Apoptosis of SHEDs was determined with Annexin V-FITC/PI double staining after incubation with or without 50 or 100 μM CoCl2 for 3 days. Flow cytometric contour plots and the percentage of apoptotic cells are shown. d The mRNA expression of Bcl-2 was examined by qRT-PCR. The data are shown as relative gene expression compared with the internal control. Bar graphs represent the mean ± SD of triplicate independent experiments.*P < 0.05 vs. control

Article Snippet: After incubation with a Dylight 650 conjugated anti-STRO-1 antibody (Novus Biologicals, USA) in the dark at 4 °C for 1 h, the cells were washed twice with PBS and then analyzed by flow cytometry.

Techniques: CCK-8 Assay, Incubation, Double Staining, Expressing, Quantitative RT-PCR